Analysis Note

Five distinct bands by SDS-PAGE

Biochem/physiol Actions

Primary TargetGuanine nucleotide-binding regulatory proteins Gi, Go, and Gt

Product does not compete with ATP.

General description

NOTE: This product is not activated, hence for use in cell-free systems, activation is required.

A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins G_i, G_o, and G_T. Affects glucose homeostasis, immune cell function, and the induction of leukocytosis. Used in the study of adenylate cyclase regulation and the role of G_i proteins. Holotoxin activity is determined in CHO cell assay. Note: this product is not activated. If used with an intact cell system, the cells will activate the toxin. If used in a cell-free system, activation may be achieved by the methods of either Kaslow, H.R., and Burns, D.L. 1992. and Moss, J. 1983.

This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.

Obtain a uniform suspension before use. A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins G_i, G_o, and G_t. Used in the study of adenylate cyclase regulation and the role of G_i proteins. Holotoxin activity is determined in a CHO cell assay.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Joshi, S.A., et al. 1998. FEBS Lett. 441, 67.Fryer, M.W. 1992. Neurosci. Lett.146, 84.Gierschik, P. 1992. Curr. Topics Microbiol. Immunol.175, 69.Harada, J., et al. 1992. Eur. J. Pharmacol.227, 301.Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem.267, 13735.Kaslow, H.R., and Burns, D.L., 1992. FASEB J.6, 2684.Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap.263, 1479.Hewlett, E.L., et al. 1983. Infect. Immun.40, 1198.Moss, J., et al. 1983. J. Biol. Chem.258, 11879.

Physical form

Lyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.

Reconstitution

Following reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.

Reconstitute with 500 µl H₂O. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.

Warning

Toxicity: Toxic (F)